You are visiting a website that is not intended for your region

The page or information you have requested is intended for an audience outside the United States. By continuing to browse you confirm that you are a non-US resident requesting access to this page or information.

Switch to the US site

Select Your Country or Region
bioreactor sterilization

Best Practices for Multi-Use Bioreactor Sterilization


Best Practices for Multi-Use Bioreactor Sterilization

Regardless of whether the bioreactor is being prepared for a same-use application or an entirely different one, all bioreactor components, as well as liquid media, must be made ready through an effective and reproducible process.

Sterilization Process

Regardless of operation mode, the autoclave must meet the minimum temperature plateau of 121°C with a minimum dwell time of 20 minutes to ensure the sterilization cycle’s efficacy.

3 test types and 3 sterilization methods

  1. Standard Test Cycle
    Steam injection heats to 121°C for a minimum of 20 minutes

  2. Pre-Vacuum
    Pre-vacuum is added to increase air removal and improve steam penetration within vessel and tubing

  3. Pre-Vacuum and Pressure Pulses
    Pre-vacuum is followed by steam pressure pulses to remove any remaining air and provide maximum steam penetration in vessel and tubing.

Empty and Full Bioreactor

To evaluate the efficacy of an extended dwell time a test was performed in Getinge Sterilization Test Lab.
Testing compared the results of a 40-minute dwell time in an empty bioreactor (CC 899) with a 3L vessel filled 2.4L (CC 900) for the same dwell time.

In CC 899, the temperature sensor placed in the thermowell still did not reach 121°C, even by the end of the 40-minute period. However, bioindicator test results indicated no bacterial growth, verifying that extended plateau time improves sterilization results.
In CC 900, the extended plateau time and reduced liquid in the vessel allowed the minimum temperature to be reached throughout. Bioindicator results also verified sterilization in this scenario.

In CC 900, the extended plateau time and reduced liquid in the vessel allowed the minimum temperature to be reached throughout. Bioindicator results also verified sterilization in this scenario.

Download the application brief here:

Best practices for Multi-use Bioreactors application brief.png

Applikon bioreactor systems

The Applikon bioreactor system portfolio covers the whole upstream bioprocess, ranging from laboratory scale and pilot plant scale to production scale.

Læs mere om Applikon bioreactor systems


Getinge’s portfolio of washers meets a broad set of cleaning applications in the Life Science industry, ranging from cGMP cleaning in biopharmaceutical production to the research space. For a variety of laboratory settings Getinge’s laboratory washers provide clean and contamination free glass and labware.

Læs mere om Decontamination


In the production space Getinge provides sterilizers for components, equipment and tubs as well as terminal sterilization. In Biomedical research and the vivarium, Getinge offers sterilization systems meeting the need for reprocessing of cages and racks. Dedicated laboratory sterilizers provide reliable processes for a variety of applications and lab requirements.

Læs mere om Sterilization

Related articles

  • Research Laboratories

5 Factors for Safe Material Transfer in BSL-3 and BSL-4 Facilities

BSL-3 and BSL-4 laboratories highlight the fact that if proper protocols are not followed, contamination can cause detrimental results.

Learn more

  • Research Laboratories

An automated bioreactor solution tailored for the next generation of science

At the Delft University of Technology in the Netherlands, professors and PhD students needed a new solution for evolutionary engineering – to improve the study of genetically engineered cells in the pursuit of replacing more petrochemicals with plant-based materials. This called for a robust, flexible, and automated bioreactor setup tailored to meet the specific challenges related to evolution studies.

Læs mere

  • Research Laboratories

Top 3 Causes of Cross Contamination in Biosafety Facilities

Cross contamination in BSL-3 and BSL-4 laboratories represents a significant risk to personnel and the surrounding community due to the dangerous agents housed within these facilities. 

Learn more

  • Research Laboratories

The 4 Pillars of Successful Labware Cleaning

Clean labware is a critical component in any laboratory because contaminated labware can lead to false test results and expensive re-testing.

Learn more

  • Research Laboratories

Bioreactor preparation solutions

An automated, preprogrammed cleaning process employs best practices for cleaning the multi-use bioreactor. This allows you to validate your cleaning process and ensure that your bioreactor is ready for sterilization.

Læs mere