High quality multi-use bioreactor for lab-scale applications
The stirred tank Applikon Bio excels in quality and modularity. This glass autoclavable bioreactor supports you in your research and development applications and smoothly optimizes your process while scaling up to higher volumes.
Easily configure your glass autoclavable bioreactor to changing process demands
- Simple set-up and easy handling
- Wide range of volumes to fit many applications
- Easy cleanability by electropolished finish of the product
- Interchangeable modules to tailor the systems to research demands
The Applikon Bio is easy to upgrade if a change in research activities occurs, whether it involves cell culture applications or microbial culture applications. Because of the modularity and flexibility, you can always adapt the system to
changing process demands. This results in low initial investment and low running costs.
The stirred tank reactor (STR) is the most widely used bioreactor type. Getinge Applikon multi-use bioreactors and fermenters are available in 2 / 3 / 5 / 7 / 15 / 20 liter total volume.
Advances in bioreactor washing
The programmable Getinge Ultima Lancer labware washer with specialty washer rack for the multi-use 1-3 L bioreactors assures thorough cleaning, better reproducibility and reduced labor.
Watch the video to view how the Getinge Applikon bioreactors and Getinge Lancer washers bring you a holistic solution for lab applications.
This Applikon bioreactor straight in your lab
Are you interested in the multi-use Applikon bioreactor, and would you like to get a virtual sample right now? Try this bioreactor in Augmented Reality*.
Scan the QR code on your desktop with your mobile device or click on the link directly on the mobile device and discover how the Applikon Bio would be on your bench.
*View this page on an Augmented Reality (AR) compatible device. Augmented Reality (AR) adds digital elements to the camera of your smartphone, creating the illusion that holographic content is a part of a physical world around you.
Features & Specifications
The glass dished bottom vessels are made of borosilicate glass to guarantee:
- resistance to thermal shock
- excellent corrosion resistance
- smooth, non porous surface for easy cleaning
- optimal transparency for visual inspection of the culture
High quality parts
The system is built according to the specific demands of a process using an extensive array of high quality components. All metal parts of the bioreactor are constructed of stainless steel 316L. There are no welded parts in the bioreactor topplate, which simplifies the sterilization of the bioreactor after each run.
Up to 0.5 barg
The glass bioreactor vessels can be used up to 0.5 barg (7.5 psig) of overpressure.
|2 liter single wall||2.2||1.7||0.5||2.2||1.9|
|2 liter jacketed||2.2||1.7||0.5||2.2||1.9|
|3 liter single wall||3.1||2.7||0.5||1.9||1.5|
|3 liter jacketed||3.1||2.7||0.5||1.9||1.5|
|5 liter single wall||4.8||3.4||0.9||1.6||1.1|
|5 liter jacketed||4.8||3.4||0.9||1.6||1.1|
|7 liter single wall||6.8||5.4||1.5||2.2||1.8|
|7 liter jacketed||6.8||5.4||1.5||2.2||1.8|
|15 liter single wall||16.5||12||3.0||1.7||1.5|
|15 liter jacketed||18.2||12||3.0||1.5||1.2|
|20 liter single wall||23.4||16||3.0||2.4||2.0|
|Internal Diameter (mm)||Internal Height (mm)||Autoclave dimensions (WxH mm)|
|2 liter single wall||105||240||ø190 x 436|
|2 liter jacketed||105||240||ø219 x 486|
|3 liter single wall||130||240||ø190 x 436|
|3 liter jacketed||130||240||ø235 x 436|
|5 liter single wall||160||250||ø260 x 436|
|5 liter jacketed||160||250||ø260 x 480|
|7 liter single wall||160||350||ø260 x 600|
|7 liter jacketed||160||350||ø264 x 645|
|15 liter single wall||222||440||ø381 x 710|
|15 liter jacketed||240||440||ø391 x 740|
|20 liter single wall||222||620||ø381 x 900|
|Drive system||Direct drive, lipsealed or magnetically coupled|
|Stirrer speed (rpm)||Standard range is 50 - 1250
2 and 3 liter systems can be supplied with 2000 rpm motor
|Impellers||Rushton and marine with outside diameters 45 mm, 60 mm 75 mm or 85 mm|
|Gas sparger||Porous sparger or L-type sparger|
|Exhaust gas||Water cooled exhaust gas condenser|
|Sampling||Fixed height or height adjustable sample pipe with optional sampling system
Sample pipe internal diameters choices are: 1.7 mm, 4 mm, 6 mm or 10 mm
|Additions||Triple or single inlet ports and optional micro liquid injectors|
|pH||Measurement: 12 mm classic pH sensor|
|Control: via acid pump or CO2 gas (rotameter or MFC) in combination with alkali pump|
|DO2||Measurement: 12 mm classic polarographic DO2 sensor or LumiSens for 2-5 L
Control: via a combination of N2, Air, O2 (Rotameter or MFC) and agitation
|Temperature||Measurement: Pt-100 sensor in thermowell in topplate
Control: cooling and/or heating jacket via bioreactor wall or via internal heat exchanger
|Foam||Measurement: Height adjustable conductivity based foam sensor
Control: anti-foam addition pump
|Level||Measurement: Height adjustable conductivity based level sensor
Control: pump for liquid addition or removal
|Optional inlets||Septum, chemostat tube, liquid entry system|
The Applikon Bio is ideal for the following applications:
- Microbial cultures
- Cell cultures
- Continous cultivation
Applikon Alcohol Fermentation Monitor
The Applikon Alcohol Fermentation Monitor (AFM) is a robust and user-friendly laboratory parallel fermentation system that allows for accurate comparisons of carbon conversion rates and yields for six simultaneous anaerobic fermentations.
An easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines. This system enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handlings.
The my-Control is ideal for small scale cultivations as this controller requires less bench space than other controllers. This advanced system can control single- and multi-use bioreactors from 50 mL (working volume) up to a total volume of 3 L for both cell culture and microbial cultures.
The ez2-Control is there to simplify your bioprocess. Its multiple software innovations make it easy to control your bioreactor. The web-interface for the ez2-Control provides a clear overview of the parameters of your bioprocess, putting the attention right where it’s needed.
Applikon Motor Controller
The Motor Controller is a robust and user-friendly device that allows for easy and stable mixing. The Motor Controller can be supplied with 4 different types of brushless stirrer motors that can support mixing in vessels up to 130 L.
The in-Control is a process controller for laboratory scale bioreactors, offering high level control on a small footprint. This modular system allows you to create the most optimal set-up as you can add any extra inputs or outputs. You are in control.
The Applikon BioSep is a unique cell retention device for the process of constant feeding of fresh media and removal of spent media, while retaining a high number of viable cells. It uses high frequency resonant ultrasonic waves to separate cells and thereby automatically removes cell debris, which makes it the perfect solution for continuous bioprocesses.
Applikon AppliFlex ST
Single-use bioreactors provide higher throughput thanks to easy setup and operation. Plus minimal operator handling reduces the ever-present risk of contamination. The Applikon AppliFlex ST has been designed to make your life in the lab easy.