Sterilize-in-Place bench scale bioreactor
The multi-use Applikon BioBench mimics commercial scale in a scale-down version, on your bench, while maintaining the geometric ratios of a commercial scale. The consistent concept of modularity, using standard modules to build the bioreactor, simplifies customization to meet any specific requirements for microbial and cell cultures.
The workhorse in your process development
- Compact design reduces floor space needed
- cGMP compliant design simplifies validation
- Modular design allows easy configuration to match process demands
- Worry-free operation; no punctured bags, no leaking of tubes and connectors
Essential in developing vaccines and medicines
Bioreactors play a key role in developing and producing life-saving vaccines and medicines. This complex process requires highly optimized, state of the art systems. With the Applikon BioBench standardized bioreactor designs, we provide ease and flexibility of customizing the vessel to handle any type of application needs with standard components.
Watch the video to view the stainless steel bioreactors being used in bioprocesses.
Features & Specifications
Easy to validate
The BioBench range is built to the latest standards on hygienic processing, meeting cGMP and GAMP requirements, and can be supplied with an extensive documentation package to support efficient validation.
Easy to operate
Stainlesss steel bioreactors minimizes the risk of contamination as there are no consumables to be replaced for every single run. Furthermore, these Sterilizable-in-Place bioreactors are supplied with magnetic coupled agitator connections, instead of traditional connections (lipsealed or double mechanical seal). Since there is no connection with the outside world, there is no risk of contamination.
The open frame construction gives easy access for maintenance and operation.
Easy to clean
The Applikon stainless steel bioreactor range is completely fabricated using pharma-grade 316L stainless steel and supplied with a surface finish of Ra < 0.4 micron. This smooth surface ensures that biofilms and debris will not stick to the internals of the bioreactors. This greatly simplifies the cleaning procedure and creates ease of mind knowing that the bioreactors will always be reproducible clean.
|Total volume(liter)||Working volume(liter)||Minimum working volume(liter)||Aspect ratio total volume||Aspect ratio working volume|
|20 liter Bio Bench||20||15||4||3.0||2.2|
|30 liter Bio Bench||30||22.5||7||2.0||1.6|
|Drive system||Magnetically coupled, optional mechanical seal, bottom or top mounted agitator for microbial cultures and top mounted for cell culture systems|
|Maximum agitator tipspeed (m/s)||5 m/s for microbial cultures and 1 m/s for cell cultures|
|Impellers||Rushton and marine with outside diameters 0.3 - 0.5 vessel diameter|
|Gas sparger||Porous sparger, L-Sparger or Ring-type sparger|
|Gas overlay||Optional gas overlay line|
|Exhaust gas||Water cooled exhaust gas condenser with internal spiral and/or jacketed|
|Sampling||Optional resterilizable sample system in DN25 port in lower side wall|
|Draining||Resterilizable bottom mounted bellows drain|
|Additions||Sterilizable additions (push valves) and resterilizable addition ports|
|pH||Measurement: 12 mm classic pH sensor in DN25 port in lower side wall|
|Control: via acid pump or CO2 gas (rotameter or MFC) in combination with alkali pump|
|DO2||Measurement: 12 mm polarographic DO2 sensor in DN25 port in lower side wall|
|Control: via a combination of N2, Air, O2 (Rotameter or MFC) and agitation|
|Temperature||Measurement: Pt-100 sensor in DN25 port in lower side wall|
|Cultivation control: cooling and heating jacket via bioreactor wall|
|Foam||Measurement: Height adjustable conductivity based foam sensor|
|Measurement: Height adjustable conductivity based foam sensor|
|Level||Measurement: Height adjustable conductivity based level sensor or loadcells in bioreactor frame|
|Control: pump for liquid addition or removal|
The Applikon BioBench is ideal for the following applications:
- Scale-up studies
- Medium optimization
- Process optimization
- Small scale production
- Seed train
- Microbial and cell culture
- Batch, Fed-Batch, Perfusion and Continuous cultivation
Applikon Alcohol Fermentation Monitor
The Applikon Alcohol Fermentation Monitor (AFM) is a robust and user-friendly laboratory parallel fermentation system that allows for accurate comparisons of carbon conversion rates and yields for six simultaneous anaerobic fermentations.
An easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines. This system enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handlings.
The my-Control is ideal for small scale cultivations as this controller requires less bench space than other controllers. This advanced system can control single- and multi-use bioreactors from 50 mL (working volume) up to a total volume of 3 L for both cell culture and microbial cultures.
The ez2-Control is there to simplify your bioprocess. Its multiple software innovations make it easy to control your bioreactor. The web-interface for the ez2-Control provides a clear overview of the parameters of your bioprocess, putting the attention right where it’s needed.
Applikon Motor Controller
The Motor Controller is a robust and user-friendly device that allows for easy and stable mixing. The Motor Controller can be supplied with 4 different types of brushless stirrer motors that can support mixing in vessels up to 130 L.
The in-Control is a process controller for laboratory scale bioreactors, offering high level control on a small footprint. This modular system allows you to create the most optimal set-up as you can add any extra inputs or outputs. You are in control.
The Applikon BioSep is a unique cell retention device for the process of constant feeding of fresh media and removal of spent media, while retaining a high number of viable cells. It uses high frequency resonant ultrasonic waves to separate cells and thereby automatically removes cell debris, which makes it the perfect solution for continuous bioprocesses.
Applikon AppliFlex ST
Single-use bioreactors provide higher throughput thanks to easy setup and operation. Plus minimal operator handling reduces the ever-present risk of contamination. The Applikon AppliFlex ST has been designed to make your life in the lab easy.