You are visiting a website that is not intended for your region

The page or information you have requested is intended for an audience outside the United States. By continuing to browse you confirm that you are a non-US resident requesting access to this page or information.

Switch to the US site

Select Your Country or Region
Send

Applikon Micro-Flask

The Applikon Micro-Flask with various configurations of cover clamps and sandwich covers
The Applikon Micro-Flask by Duetz design

Applikon Micro-Flask

Reproducible cultivation in microtiter plates

An easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines. This system enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handlings.

Oversikt

Speed up your process by cultivating strains in parallel

  • Low and uniform evaporation rates
  • Sterile barrier for individual wells prevents cross contamination
  • Oxygen transfer rates similar to shake flasks in standard orbital shakers
  • Conversion of 24 and 96 microtiter plates (both deep and low-well plates) into individual micro-bioreactors
  • Simultaneous and reproducible sampling of 96 frozen glycerol stocks using our Cryo-Replicator

All handling with the Micro-Flask is performed in parallel with sets of 24 or 96 strains. It provides a more efficient use of your current orbital shaker, with time savings up to a factor 10 possible. It allows for the same quality of growth as in Erlenmeyer flasks in terms of oxygen transfer rates and reproducibility.

Consumables & Accessories

The Applikon Micro-Flask allows for:

  • various square deep-well and round low-well microtiter plates
  • a Sandwich-cover (silicone seal, ePTFE + microfibre layer and stainless steel cover)
  • a clamp
  • a Cryo replicator and press

Features & Specifications

Individual micro-reactors

The Micro-Flask turns 6, 24 and 96-well microtiter plates into individual micro-reactors enabling simultaneous and reproducible cultivations.

Transfer of oxygen

The oxygen transfer in the Micro-Flask is similar to fermenters enabling accurate cultivations.

Specifications

Type of
microtiter plate
Well volume Culture volume Orbital shaking frequency Shaking amplitude O2-transfer rate
(30˚C, air, 1 bar)
Headspace refreshment rate Evaporation rate per well
(at 30˚C)
Mixing pattern
at 300 rpm
             

 

ampl.
25 mm
ampl.
50 mm
24-square deep-well
polypropylene,
17x17 mm,
depth 40 mm
11000 µL 2500 µL 300 rpm 50 mm 51 mmol O2/l/h 2.5 mL / min
(1 VVM)

50% humidity:
50 µL H2O per day

75% humidity:
25 µL H2O per day

2500 µL 2500 µL
2500 µL 300 rpm 25 mm 39 mmol O2/l/h
2500 µL 220 rpm 50 mm 35 mmol O2/l/h
         
4000 µL 300 rpm 50 mm 24 mmol O2/l/h 2.5 mL / min
(0.6 VVM)
4000 µL 220 rpm 25 mm 24 mmol O2/l/h
24-round low-well
polystyrene, ⌀16 mm,
depth 18 mm
3000 µL 750 µL 300 rpm 50 mm 40 mmol O2/l/h 1.1 mL / min
(1.4 VVM)

50% humidity:
30 µL H2O per day

75% humidity:
15 µL H2O per day

1000 µL 1000 µL
750 µL 300 rpm 25 mm 25 mmol O2/l/h
         
1000 µL 300 rpm 50 mm 30 mmol O2/l/h 1.1 mL / min
(1.1 VVM)
1000 µL 300 rpm 25 mm 19 mmol O2/l/h
96-square deep-well
polypropylene,
8x8 mm,
depth 40 mm
2400 µL 500 µL 300 rpm 50 mm 38 mmol O2/l/h 1 mL / min
(2 VVM)

50% humidity:
22 µL H2O per day

75% humidity:
11 µL H2O per day

750 µL 750 µL
500 µL 300 rpm 25 mm 12 mmol O2/l/h
         
750 µL 300 rpm 50 mm 24 mmol O2/l/h 1 mL / min
(1.3 VVM)
750 µL 300 rpm 25 mm 7 mmol O2/l/h
         
1000 µL 300 rpm 50 mm 18 mmol O2/l/h 1 mL / min
(1 VVM)
1000 µL 300 rpm 25 mm 3 mmol O2/l/h
96-round low-well
polystyrene,
⌀6.5 mm,
depth 11 mm
380 µL 100 µL 300 rpm 50 mm 39 mmol O2/l/h 250 µL / min
(2.5 VVM)

50% humidity:
6 µL H2O per day

75% humidity:
3 µL H2O per day

150 µL 150 µL
100 µL 300 rpm 25 mm 20 mmol O2/l/h
         
150 µL 300 rpm 50 mm 32 mmol O2/l/h 250 µL / min
(1.7 VVM)
150 µL 300 rpm 25 mm 16 mmol O2/l/h
         
200 µL 220 rpm 50 mm 12 mmol O2/l/h 250 µL / min
(1.3 VVM)
200 µL 300 rpm 25 mm 12 mmol O2/l/h

 

Applications

The Applikon Micro-Flask is ideal for the following applications:

  • Growth medium optimization studies
  • Comparative studies e.g. clinical isolates
  • Screening and distribution of clone libraries in E. coli or yeasts
  • High-throughput screening for high-activity or high-productivity mutants
  • High-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)

Relaterte produkter

Applikon Alcohol Fermentation Monitor

The Applikon Alcohol Fermentation Monitor (AFM) is a robust and user-friendly laboratory parallel fermentation system that allows for accurate comparisons of carbon conversion rates and yields for six simultaneous anaerobic fermentations.

Lær mer

Applikon my-Control

The my-Control is ideal for small scale cultivations as this controller requires less bench space than other controllers. This advanced system can control single- and multi-use bioreactors from 50 mL (working volume) up to a total volume of 3 L for both cell culture and microbial cultures.

Lær mer

Applikon ez2-Control

The ez2-Control is there to simplify your bioprocess. Its multiple software innovations make it easy to control your bioreactor. The web-interface for the ez2-Control provides a clear overview of the parameters of your bioprocess, putting the attention right where it’s needed.

Lær mer

Applikon Motor Controller

The Motor Controller is a robust and user-friendly device that allows for easy and stable mixing. The Motor Controller can be supplied with 4 different types of brushless stirrer motors that can support mixing in vessels up to 130 L.

Lær mer

Applikon in-Control

The in-Control is a process controller for laboratory scale bioreactors, offering high level control on a small footprint. This modular system allows you to create the most optimal set-up as you can add any extra inputs or outputs. You are in control.

Lær mer

Applikon BioSep

The Applikon BioSep is a unique cell retention device for the process of constant feeding of fresh media and removal of spent media, while retaining a high number of viable cells. It uses high frequency resonant ultrasonic waves to separate cells and thereby automatically removes cell debris, which makes it the perfect solution for continuous bioprocesses.

Lær mer

Applikon AppliFlex ST

Single-use bioreactors provide higher throughput thanks to easy setup and operation. Plus minimal operator handling reduces the ever-present risk of contamination. The Applikon AppliFlex ST has been designed to make your life in the lab easy.

Lær mer

Applikon MiniBio

The Applikon MiniBio is a true scale down of the classic laboratory scale bioreactor. It has the same flexibility, which means that the Applikon MiniBio can be customized to fit the demands of any process. The small volume reduces media costs and maximizes usage of bench space.

Lær mer