Reproducible cultivation in microtiter plates
An easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines. This system enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handlings.
Speed up your process by cultivating strains in parallel
- Low and uniform evaporation rates
- Sterile barrier for individual wells prevents cross contamination
- Oxygen transfer rates similar to shake flasks in standard orbital shakers
- Conversion of 24 and 96 microtiter plates (both deep and low-well plates) into individual micro-bioreactors
- Simultaneous and reproducible sampling of 96 frozen glycerol stocks using our Cryo-Replicator
All handling with the Micro-Flask is performed in parallel with sets of 24 or 96 strains. It provides a more efficient use of your current orbital shaker, with time savings up to a factor 10 possible. It allows for the same quality of growth as in Erlenmeyer flasks in terms of oxygen transfer rates and reproducibility.
Consumables & Accessories
The Applikon Micro-Flask allows for:
- various square deep-well and round low-well microtiter plates
- a Sandwich-cover (silicone seal, ePTFE + microfibre layer and stainless steel cover)
- a clamp
- a Cryo replicator and press
Features & Specifications
|Well volume||Culture volume||Orbital shaking frequency||Shaking amplitude||O2-transfer rate
(30˚C, air, 1 bar)
|Headspace refreshment rate||Evaporation rate per well
at 300 rpm
depth 40 mm
|11000 µL||2500 µL||300 rpm||50 mm||51 mmol O2/l/h||2.5 mL / min
|2500 µL||2500 µL|
|2500 µL||300 rpm||25 mm||39 mmol O2/l/h|
|2500 µL||220 rpm||50 mm||35 mmol O2/l/h|
|4000 µL||300 rpm||50 mm||24 mmol O2/l/h||2.5 mL / min
|4000 µL||220 rpm||25 mm||24 mmol O2/l/h|
polystyrene, ⌀16 mm,
depth 18 mm
|3000 µL||750 µL||300 rpm||50 mm||40 mmol O2/l/h||1.1 mL / min
|1000 µL||1000 µL|
|750 µL||300 rpm||25 mm||25 mmol O2/l/h|
|1000 µL||300 rpm||50 mm||30 mmol O2/l/h||1.1 mL / min
|1000 µL||300 rpm||25 mm||19 mmol O2/l/h|
depth 40 mm
|2400 µL||500 µL||300 rpm||50 mm||38 mmol O2/l/h||1 mL / min
|750 µL||750 µL|
|500 µL||300 rpm||25 mm||12 mmol O2/l/h|
|750 µL||300 rpm||50 mm||24 mmol O2/l/h||1 mL / min
|750 µL||300 rpm||25 mm||7 mmol O2/l/h|
|1000 µL||300 rpm||50 mm||18 mmol O2/l/h||1 mL / min
|1000 µL||300 rpm||25 mm||3 mmol O2/l/h|
depth 11 mm
|380 µL||100 µL||300 rpm||50 mm||39 mmol O2/l/h||250 µL / min
|150 µL||150 µL|
|100 µL||300 rpm||25 mm||20 mmol O2/l/h|
|150 µL||300 rpm||50 mm||32 mmol O2/l/h||250 µL / min
|150 µL||300 rpm||25 mm||16 mmol O2/l/h|
|200 µL||220 rpm||50 mm||12 mmol O2/l/h||250 µL / min
|200 µL||300 rpm||25 mm||12 mmol O2/l/h|
The Applikon Micro-Flask is ideal for the following applications:
- Growth medium optimization studies
- Comparative studies e.g. clinical isolates
- Screening and distribution of clone libraries in E. coli or yeasts
- High-throughput screening for high-activity or high-productivity mutants
- High-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)
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