Customized solutions to suit your bioprocessing needs
As conditions for optimal growth of cells and microbial organisms differ per application, the design of the bioreactor or fermentor differs as well. The BioProduction stands for special projects, up to complete cGMP production systems of several thousand liters, that include tailor-made systems to match cultivation requirements.
Built for your needs and application
- Complete turnkey system with automation
- Wide choice of controllers with many PLC and DCS options
- Design, fabrication and automation based on collaborative approaches and project managed with your inputs at every stage
The processing steps towards building the most optimal cultivation system can be complex, requiring tailer-made systems. Using modern design technologies, we will guide you through the process - from conception to installation. Our systems can be configured to various applications, whether microbial or cell cultures, and can be customized to specific requirements like custom volumes, cleaning solutions, access platforms, control solutions and materials.
Choose the best options for your goals; skids on wheels or permanent mounts, mobile or stationary Clean-In-Place with or without automation. Complementary media prep, harvest and inactivation systems can be supplied as well. Design any system to suit your needs.
Essential in producing vaccines and medicines
Bioreactors play a key role in producing life-saving vaccines and medicines. This complex process requires highly optimized, state of the art systems. With the Applikon BioProduction, we provide ease and flexibility of customizing the vessels to handle any type of application needs.
Watch the video to view the stainless steel bioreactors being used in bioprocesses.
Features & Specifications
Easy to validate
The stainless steel bioreactors and fermenters are built to the latest standards on hygienic processing, meeting cGMP and GAMP requirements, and can be supplied with an extensive documentation package to support efficient validation.
Easy to operate
Stainlesss steel bioreactors minimizes the risk of contamination as there are no consumables to be replaced for every single run. Furthermore, these Sterilizable-in-Place bioreactors are supplied with magnetic coupled agitator connections. Whereas the agitator shaft continues from the outside to the inside of the bioreactor with traditional connections (lipsealed or double mechanical seal), here the connection is established via magnets. Since there is no connection with the outside world, there is no risk of contamination.
The open frame construction gives easy access for maintenance and operation.
Easy to clean
The Applikon stainless steel bioreactor range is completely fabricated using pharma-grade 316L stainless steel and supplied with a surface finish of Ra < 0.4 micron. This smooth surface ensures that biofilms and debris will not stick to the internals of the bioreactors. This greatly simplifies the cleaning procedure and creates ease of mind knowing that the bioreactors will always be reproducible clean.
|Total volume||Custom built bioreactor systems are available up to 5,000 liter total volume|
|Drive system||Magnetically coupled, mechanical seal, bottom or top mounted agitator options available systems|
|Maximum agitator tipspeed (m/s)||up to 5 m/s|
|Impellers||Rushton, marine, hydrofoil and more|
|Gas sparger||Porous sparger, L-Sparger or Ring-type sparger|
|Gas overlay||Optional gas overlay line|
|Exhaust gas||Water cooled exhaust gas condenser with internal spiral and/or jacketed|
|Sampling||Resterilizable sample system; manual or fully automated SIP and CIP possible|
|Draining||Resterilizable bottom mounted drain; manual or fully automated SIP and CIP possible|
|Additions||Sterilizable additions (push valves) and resterilizable addition ports; fully automated SIP and CIP possible|
|pH||Measurement: 12 mm classic pH sensor in DN25 port in lower side wall, redundant pH sensor possible|
|Control: via acid pump or CO2 gas (rotameter or MFC) in combination with alkali pump|
|DO2||Measurement: 12 mm polarographic DO2 sensor in DN25 port in lower side wall, redundant DO2 sensor possible|
|Control: via a combination of N2, Air, O2 (Rotameter or MFC) and agitation|
|Temperature||Measurement: Pt-100 sensor in DN25 port in lower side wall|
|Control: cooling and heating jacket via bioreactor wall via tap water, chilled water, indirect steam or direct steam injection|
|Foam||Measurement: Height adjustable conductivity based foam sensor and/or radar foam detection|
|Control: via antifoam pump, mechanical foam breaker and/or centrifuge removal|
|Level||Measurement: Height adjustable conductivity based level sensor, radar level detection and/or loadcells under bioreactor (frame)|
|Control: pump for liquid addition or removal|
|Above mentioned options are options we can apply and have applied on our BioProduction systems in the past. Is your desired specification not listed? Please contact us and we will check options to support you building your optimal bioreactor system.|
The Applikon BioProduction is ideal for the following applications:
- Microbial and cell culture
- Batch, Fed-Batch, Perfusion and Continuous cultivation
Applikon V-Control is a sole software platform for process control, process automation and data acquisition from research to production. It enables seamless transfer of data and technology to optimize bioprocesses.
The Applikon BioSep is a unique cell retention device for the process of constant feeding of fresh media and removal of spent media, while retaining a high number of viable cells. It uses high frequency resonant ultrasonic waves to separate cells and thereby automatically removes cell debris, which makes it the perfect solution for continuous bioprocesses.
Applikon AppliSens sensors
In the biotech- and pharmaceutical industry it is vital to have precise information about the process values of the bioprocess. These values directly affect viability, productivity, stability of the cells and influence the analysis of active ingredients. The AppliSens sensors are specifically designed for long-term, stable and accurate measurements in bioprocesses.
The multi-use Applikon BioPilot is the first step towards bioprocess production. The BioPilot is linearly scaled up from lab-scale bioreactors, which simplifies the transfer to production resulting in a quicker time-to-market. The consistent concept of modularity, using standard modules to build the bioreactor, simplifies customization to meet any specific requirements for microbial and cell cultures.