Reproducible cultivation in microtiter plates
An easy-to-use, and cost-effective technology platform for the rapid handling and growth of large numbers of microbial strains, clonal libraries, mutant banks, and cell lines. This system enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handlings.
Speed up your process by cultivating strains in parallel
- Low and uniform evaporation rates
- Sterile barrier for individual wells prevents cross contamination
- Oxygen transfer rates similar to shake flasks in standard orbital shakers
- Conversion of 24 and 96 microtiter plates (both deep and low-well plates) into individual micro-bioreactors
- Simultaneous and reproducible sampling of 96 frozen glycerol stocks using our Cryo-Replicator
All handling with the Micro-Flask is performed in parallel with sets of 24 or 96 strains. It provides a more efficient use of your current orbital shaker, with time savings up to a factor 10 possible. It allows for the same quality of growth as in Erlenmeyer flasks in terms of oxygen transfer rates and reproducibility.
Consumables & Accessories
The Applikon Micro-Flask allows for:
- various square deep-well and round low-well microtiter plates
- a Sandwich-cover (silicone seal, ePTFE + microfibre layer and stainless steel cover)
- a clamp
- a Cryo replicator and press
Features & Specifications
|Well volume||Culture volume||Orbital shaking frequency||Shaking amplitude||O2-transfer rate
(30˚C, air, 1 bar)
|Headspace refreshment rate||Evaporation rate per well
at 300 rpm
depth 40 mm
|11000 µL||2500 µL||300 rpm||50 mm||51 mmol O2/l/h||2.5 mL / min
|2500 µL||2500 µL|
|2500 µL||300 rpm||25 mm||39 mmol O2/l/h|
|2500 µL||220 rpm||50 mm||35 mmol O2/l/h|
|4000 µL||300 rpm||50 mm||24 mmol O2/l/h||2.5 mL / min
|4000 µL||220 rpm||25 mm||24 mmol O2/l/h|
polystyrene, ⌀16 mm,
depth 18 mm
|3000 µL||750 µL||300 rpm||50 mm||40 mmol O2/l/h||1.1 mL / min
|1000 µL||1000 µL|
|750 µL||300 rpm||25 mm||25 mmol O2/l/h|
|1000 µL||300 rpm||50 mm||30 mmol O2/l/h||1.1 mL / min
|1000 µL||300 rpm||25 mm||19 mmol O2/l/h|
depth 40 mm
|2400 µL||500 µL||300 rpm||50 mm||38 mmol O2/l/h||1 mL / min
|750 µL||750 µL|
|500 µL||300 rpm||25 mm||12 mmol O2/l/h|
|750 µL||300 rpm||50 mm||24 mmol O2/l/h||1 mL / min
|750 µL||300 rpm||25 mm||7 mmol O2/l/h|
|1000 µL||300 rpm||50 mm||18 mmol O2/l/h||1 mL / min
|1000 µL||300 rpm||25 mm||3 mmol O2/l/h|
depth 11 mm
|380 µL||100 µL||300 rpm||50 mm||39 mmol O2/l/h||250 µL / min
|150 µL||150 µL|
|100 µL||300 rpm||25 mm||20 mmol O2/l/h|
|150 µL||300 rpm||50 mm||32 mmol O2/l/h||250 µL / min
|150 µL||300 rpm||25 mm||16 mmol O2/l/h|
|200 µL||220 rpm||50 mm||12 mmol O2/l/h||250 µL / min
|200 µL||300 rpm||25 mm||12 mmol O2/l/h|
The Applikon Micro-Flask is ideal for the following applications:
- Growth medium optimization studies
- Comparative studies e.g. clinical isolates
- Screening and distribution of clone libraries in E. coli or yeasts
- High-throughput screening for high-activity or high-productivity mutants
- High-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)
The Livit Flex is the intuitive controller that fits any research and development application. It can be configured as a single or dual control system for single-use and / or multi-use bioreactors. Only one network is needed to monitor and control the data of all your bioprocesses.
Applikon Alcohol Fermentation Monitor
The Applikon Alcohol Fermentation Monitor (AFM) is a robust and user-friendly laboratory parallel fermentation system that allows for accurate comparisons of carbon conversion rates and yields for six simultaneous anaerobic fermentations.
The my-Control is ideal for small scale cultivations as this controller requires less bench space than other controllers. This advanced system can control single- and multi-use bioreactors from 50 mL (working volume) up to a total volume of 3 L for both cell culture and microbial cultures.
The ez2-Control is there to simplify your bioprocess. Its multiple software innovations make it easy to control your bioreactor. The web-interface for the ez2-Control provides a clear overview of the parameters of your bioprocess, putting the attention right where it’s needed.
Applikon Motor Controller
The Motor Controller is a robust and user-friendly device that allows for easy and stable mixing. The Motor Controller can be supplied with 4 different types of brushless stirrer motors that can support mixing in vessels up to 130 L.
The in-Control is a process controller for laboratory scale bioreactors, offering high level control on a small footprint. This modular system allows you to create the most optimal set-up as you can add any extra inputs or outputs. You are in control.
The Applikon BioSep is a unique cell retention device for the process of constant feeding of fresh media and removal of spent media, while retaining a high number of viable cells. It uses high frequency resonant ultrasonic waves to separate cells and thereby automatically removes cell debris, which makes it the perfect solution for continuous bioprocesses.
Applikon AppliFlex ST
Single-use bioreactors provide higher throughput thanks to easy setup and operation. Plus minimal operator handling reduces the ever-present risk of contamination. The Applikon AppliFlex ST has been designed to make your life in the lab easy.