The Applikon MiniBio is a true scale down of the classic laboratory scale bioreactor. It has the same flexibility, which means that the Applikon MiniBio can be customized to fit the demands of any process. The small volume reduces media costs and maximizes usage of bench space.
Real Small... Real Bioreactors
- Easy set up and operation
- Little bench space required
- Less medium used during cultivation
- Generate more data in less time
The MiniBio is the perfect device for small-scale operations in the lab. It saves time, takes up less bench space, and generates more data while providing fully scalable results. In combination with our highly efficient BioSep cell separation device, you can convert your batch or fed-batch bioreactor into a small scale continuous bioprocessing system.
The configurable head plate of the bioreactor has Luer fittings that free up space for multiple additions, sensors and fittings and ready-to-go tubing assemblies for a quick start.
Microbial and cell cultures at small scale
Sometimes less leads to more.
The Applikon MiniBio requires less medium and bench space than regular laboratory-scale bioreactors. Scientists can easily run multiple experiments in parallel, generating more data at once to enable efficient scale-up.
Watch the video to view how the MiniBio can speed up your bioprocess.
Features & Specifications
Small size bioreactors
The compact design of the MiniBio allows the scientist to place multiple systems on the valuable bench space. Parallel processing has never been that easy.
Generate scalable results
Running cultivations at small scale allows for quick access to more data. Scientists will have reliable data that simplifies scale-up to higher volumes.
|Total volume (mL)||290||550||1250|
|Working volume (mL)||200||400||1000|
|Minimum working volume (mL)||50||100||200|
|Aspect ratio total volume||2.3||2.1||2.3|
|Aspect ratio working volume||1.6||1.5||1.9|
|Dimensions (dxh)||180 x 400 mm||195 x 400 mm||200 x 550 mm|
|Dimensions for autoclaving (dxh)||180 x 250 mm||195 x 250 mm||200 x 400 mm|
|Drive system||Direct drive, lipsealed||Direct drive, lipsealed||Direct drive, lipsealed|
|Maximum stirrer speed (rpm)||50 - 2000||50 - 2000||50 - 1750|
|Maximum impeller tip speed (m/s)||2.3||2.9||3.5|
|Impellers||Choice of Rushton and Marine|
|Gas sparger||Porous sparger, open pipe sparger or jet sparger|
|Exhaust gas||Electrically cooled exhaust gas condenser (evaporation <4% per day at 37°C @ 2vvm)|
|Sampling||Fixed sample pipe with optional sampling system|
|Draining||Height adjustable drain pipe|
|Additions||4 fixed inlet ports and optional micro liquid injectors|
|pH||Measurement: 8 mm classic pH sensor
Control: via acid pump (variable speed pump) or CO2 gas
in combination with alkali pump (variable speed pump)
|DO2||Measurement: LumiSens Optical DO2 sensor.
Control: via a combination of N2, Air, O2 (needle valve standard,
MFC optional) and agitation
|Temperature||Measurement: Pt-100 sensor in thermowell in topplate
Control: electrical cooling and heating jacket via bioreactor wall
|Foam||Measurement: Height adjustable conductivity based foam sensor
Control: anti-foam addition (variable speed pump)
|Level||Measurement: Height adjustable conductivity based level sensor
Control: variable speed pump for liquid addition or removal
|Optional inlets||Septum, chemostat tube, liquid entry system|
|Optional sensors||Biomass, Optical Density, O2 and CO2 off gas, weight balance|
The Applikon MiniBio is ideal for the following applications:
- Screening studies
- Media optimization
- Process optimization
- Microbial and cell culture
- Batch, Fed-Batch, Perfusion and Continuous cultivation
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Applikon Motor Controller
The Motor Controller is a robust and user-friendly device that allows for easy and stable mixing. The Motor Controller can be supplied with 4 different types of brushless stirrer motors that can support mixing in vessels up to 130 L.
The in-Control is a process controller for laboratory scale bioreactors, offering high level control on a small footprint. This modular system allows you to create the most optimal set-up as you can add any extra inputs or outputs. You are in control.
The Applikon BioSep is a unique cell retention device for the process of constant feeding of fresh media and removal of spent media, while retaining a high number of viable cells. It uses high frequency resonant ultrasonic waves to separate cells and thereby automatically removes cell debris, which makes it the perfect solution for continuous bioprocesses.
Applikon AppliFlex ST
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