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Applikon BioBench

The Applikon BioBench 15 liter benchtop stainless steel bioreactor with ez2-Control

Applikon BioBench

Sterilize-in-Place bench scale bioreactor

The multi-use Applikon BioBench mimics commercial scale in a scale-down version, on your bench, while maintaining the geometric ratios of a commercial scale. The consistent concept of modularity, using standard modules to build the bioreactor, simplifies customization to meet any specific requirements for microbial and cell cultures.

Overview

The workhorse in your process development

  • Compact design reduces floor space needed
  • cGMP compliant design simplifies validation
  • Modular design allows easy configuration to match process demands
  • Worry-free operation; no punctured bags, no leaking of tubes and connectors

Essential in developing vaccines and medicines

Bioreactors play a key role in developing and producing life-saving vaccines and medicines. This complex process requires highly optimized, state of the art systems. With the Applikon BioBench standardized bioreactor designs, we provide ease and flexibility of customizing the vessel to handle any type of application needs with standard components.

Watch the video to view the stainless steel bioreactors being used in bioprocesses.

Features & Specifications

Easy to validate

The BioBench range is built to the latest standards on hygienic processing, meeting cGMP and GAMP requirements, and can be supplied with an extensive documentation package to support efficient validation.

Easy to operate

Stainlesss steel bioreactors minimizes the risk of contamination as there are no consumables to be replaced for every single run. Furthermore, these Sterilizable-in-Place bioreactors are supplied with magnetic coupled agitator connections, instead of traditional connections (lipsealed or double mechanical seal). Since there is no connection with the outside world, there is no risk of contamination.

The open frame construction gives easy access for maintenance and operation.

Easy to clean

The Applikon stainless steel bioreactor range is completely fabricated using pharma-grade 316L stainless steel and supplied with a surface finish of Ra < 0.4 micron. This smooth surface ensures that biofilms and debris will not stick to the internals of the bioreactors. This greatly simplifies the cleaning procedure and creates ease of mind knowing that the bioreactors will always be reproducible clean.

Specifications

  Total volume(liter) Working volume(liter) Minimum working volume(liter) Aspect ratio total volume Aspect ratio working volume
20 liter Bio Bench 20 15 4 3.0 2.2
30 liter Bio Bench 30 22.5 7 2.0 1.6
Drive system Magnetically coupled, optional mechanical seal, bottom or top mounted agitator for microbial cultures and top mounted for cell culture systems
Maximum agitator tipspeed (m/s) 5 m/s for microbial cultures and 1 m/s for cell cultures
Impellers Rushton and marine with outside diameters 0.3 - 0.5 vessel diameter
Gas sparger Porous sparger, L-Sparger or Ring-type sparger
Gas overlay Optional gas overlay line
Exhaust gas Water cooled exhaust gas condenser with internal spiral and/or jacketed
Sampling Optional resterilizable sample system in DN25 port in lower side wall
Draining Resterilizable bottom mounted bellows drain
Additions Sterilizable additions (push valves) and resterilizable addition ports
pH Measurement: 12 mm classic pH sensor in DN25 port in lower side wall
  Control: via acid pump or CO2 gas (rotameter or MFC) in combination with alkali pump
DO2 Measurement: 12 mm polarographic DO2 sensor in DN25 port in lower side wall
  Control: via a combination of N2, Air, O2 (Rotameter or MFC) and agitation
Temperature Measurement: Pt-100 sensor in DN25 port in lower side wall
  Cultivation control: cooling and heating jacket via bioreactor wall
Foam Measurement: Height adjustable conductivity based foam sensor
  Measurement: Height adjustable conductivity based foam sensor
Level Measurement: Height adjustable conductivity based level sensor or loadcells in bioreactor frame
  Control: pump for liquid addition or removal

Applications

The Applikon BioBench is ideal for the following applications:

  • Scale-up studies
  • Medium optimization
  • Process optimization
  • Small scale production
  • Seed train
  • Microbial and cell culture
  • Batch, Fed-Batch, Perfusion and Continuous cultivation

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